Radioimmunoassay of Human Pepsinogen A and Pepsinogen C

Radioimmunoassay of human pepsinogen A and pepsinogen C.

We describe the development of radioimmunoassays to measure both human pepsinogen A and pepsinogen C concentrations in serum. The antibodies were raised in goats by immunization with purified pepsinogen A or C. The affinity constants of the respective antibodies were 20.10(10) l/mol and 7.10(10) l/mol. Pepsinogens A and C were labeled with Na 125I by the chloramine T method. The binding between...

Development of a Radioimmunoassay for Bovine Pepsinogen A

Gastric aspartic proteases (AP) are among proteolytic enzymes that are widely distributed in vertebrates. They are responsible for the digestion of dietary proteins and are synthesized as inactive precursors (prochymosin, pepsinogen and progastricsin), commonly known as zymogens. One part of their secretion by mucous neck and chief cells in the gastric mucosa (2, 22, 23) reaches the blood strea...

Estimation of serum pepsinogen.

Primary structure of human pepsinogen gene.

A recombinant clone, which covers the pepsinogen gene in a single insert, has been isolated by screening a library of human genomic DNA, using a swine pepsinogen cDNA as a probe. Sequence analysis of coding DNA segments of the clone revealed that the pepsinogen gene occupies approximately 9.4-kilobase pairs of the genomic DNA and is separated into nine exons by eight introns of various lengths....

Pepsinogen and Pepsin

Evidence relating to the structure and properties of swine pepsinogen and pepsin has been reviewed and used to suggest a tentative two dimensional picture of the skeleton of these two proteins. When pepsinogen, a folded single peptide chain, is converted to pepsin, there is a profound change in the physical and chemical properties of the protein. In an as yet unknown manner, except that it is i...